<i>In vitro</i> experimental validation of “tubulin complex assembly” biological process predictions.

(A) In vitro inhibition of tubulin polymerization by compounds predicted to perturb “tubulin complex assembly” (FDR Vmax values reflecting the maximum rate of tubulin polymerization for each compound from independent replicate experiments are plotted. Assay positive and negative control compounds are colored grey. (B) Structural similarity-based hierarchical clustering of compounds tested in (A). Single linkage was used in combination with (1 –structural similarity) as the distance metric; as such, the structural similarity of the two most similar compounds at each junction can be inferred directly from the dendrogram. Compounds predicted to perturb “tubulin complex assembly” (FDR Materials and Methods).